Molecular diversity analysis among elite genotypes of American cotton (Gossypium hirsutum L.)Pantnagar Journal of Research, Volume - 18, Issue - 1 ( January-April, 2020)
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Cotton is the most important fiber crop globally, grown over tropical and subtropical regions world. The narrow genetic base of the cotton germplasm being used in breeding programs is one of the factors in failing to achieve appreciable amount of progress in improving fiber as well as yield contributing traits. Molecular markers have been successfully applied to estimate genetic diversity, besides their use in advancing the breeding cycle. ISSR markers were used to assess molecular diversity among nine elite genotypes in upland cotton in this investigation. Primers ISSR 32 and UBC 842 were recorded with maximum PIC value (0.68) indicating maximum contribution of these primers for diversity analysis. The NTSYS-pc UPGMA tree cluster analysis resulted that nine genotypes were quite distinct and were grouped in four clusters at 69% similarity index. The use of two genotypes from different clusters in hybridization could produce feasible heterotic combinations in forthcoming breeding programmes.
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