Formerly International Journal of Basic and Applied Agricultural Research

Plumule colouration as a criterion to improve the efficiency of R1-nj marker based doubled haploid breeding in maize

PRABHAT SINGH, MUKESH KUMAR KARNWAL, SMRUTISHREE SAHOO, ARVIND CHAUHAN and NARENDRA KUMAR SINGH
Pantnagar Journal of Research, Volume - 20, Issue - 2 ( May-August 2022)

Published: 2022-08-31

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Abstract


The experiment was conducted using eight source populations comprised of F1s, F2s, landrace and composite genotype of both field corn and sweet corn and these genotypes were crossed as a female parent with CIMMYT bred tropicalized advance haploid inducer line possessing R1-nj colour marker gene. Based on the colour marker of endosperm as well as endosperm and embryo, the seeds were classified into contaminants, haploids and diploids. The haploid kernels were then categorized based on plumule coloration and no plumule colouration after in vitro germination. Plumule coloured seedlings were directly grown in the field by hypothesizing that these seedlings are similar to diploids but the confirmations were still needed. The remaining seedlings that did not have plumule colour were diploidized using colchicines (0.04%) after cutting shoot and root tips of the in vitro germinated seeds. The seedlings having colour on plumule exhibited differential segregation on kernels after selfing due to R1-nj background effect whereas seedlings without plumule colour showed significant haploid behaviour. The seedlings sorted from haploids after germination on the basis of plumule colour was highest in PSM-3 (22) followed by Landrace DL1 (19) whereas the misclassification percentage was highest of 25% in Landrace DL1 followed by PSM-3 (18%) and sweet corn F1 ’s (13%). The results therefore showed that second line of sorting using plumule colour in germinated seedlings has significant impact on reducing false doubled haploids and thereby increasing resource use efficiency in doubled haploid breeding program


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