Pantnagar Journal of Research

Abstract

This study was undertaken for genetic diversity and bottleneck analysis in Udaipuri goat found in Pauri Garhwal district of Uttarakhand using a set of twenty microsatellite markers. Blood samples were collected from 30 unrelated animals from the breeding tract. All the twenty microsatellite loci were amplified successfully and a total of 96 alleles were observed across all the loci. When bottleneck analysis was performed, sign test revealed the observed heterozygosity excess was higher than the expected utilizing IPM, TPM and SMM. The expected heterozygosity (Hee) values were 11.31, 11.66 and 11.85 while the observed heterozygosity (He) were 20, 18 and 14 for IAM, TPM and SMM, respectively. Under standardized test, T2 values were positive (4.958, 3.766 and 2.032 utilizing the IAM, TPM and SMM, respectively) indicating a gene diversity excess that is caused by reduction in effective population size.