Pantnagar Journal of Research

Abstract

Animals and man are inadvertently exposed to various man-made or natural forms of nano particles due to their presence in the micro-environment. Limited investigations have been carried on effect of aluminium nanoparticles on immune response and health status of animals and man. Therefore, present study was designed to study the effect of aluminium nano particles on humoral immune response of Wistar rats. For this study, a total of 35 rats of six-week age were divided randomly into two groups of 20 rats in Group I (control) and 15 rats in Group II (treated). The control group fed with normal standard recommended feed while treated group exposed to aluminium nano particles orally at NOAEL dose viz.6 mg/kg BW per day for a period of 90 days. Humoral immune response was assessed by hemagglutination (HA) and hemagglutination inhibition (HI) assays and also by performing ELISA from the serum separated from blood of 5 rats from each group at 30th, 60th and 90th DPT. The antigen used was reconstituted commercial Ranikhet Disease Vaccine, R2B strain vaccine Mukteshwar (IndovaxPvt. Ltd., Hissar). There was increase in humoral immune response in treated group but the results were not significant statistically.